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UK Stem Cell Bank
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Cell Lines Catalogue
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Research grade stem cell lines
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Laboratory Grade Stem Cell Lines
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NCL-4
NCL-4
Cell Line Name
NCL-4
Cell Line Status
Currently available
Cell Type
Human Embryonic
Grade
Research
Source
Human embryo / blastocyst
Source type
Fresh
Consent Status
Donor consent was reviewed by the UK Steering Committee and was found to be consistent with Human Fertilisation and Embryology Authority (HFEA) guidelines for centres deriving human embryonic stem cell lines under HFEA license and the requirements of UK le
Date cell line derived
30 October 2004
Ethical Statement
In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor
Newcastle Centre for Life
Depositing Centre
Newcastle Fertility Centre at LIFE
Country of Origin
United Kingdom
MUL Contact Details
legal@nesci.ac.uk
Journal Reference
Derivation of hes cells from developing and arrested embryos. Zhang X, StojkovicM et al. Stem Cells 2006 Dec; 24(12) 2669-2676
SCSC No.
SCSC-06-46(3)
UKSCB Accession No.
R-07-016
Distribution Bank No.
D01
Passage No.
p28
Feeder Cell Type
Mouse embryo fibroblast
Basel Medium
KO-DMEM
Protein Source
KO-SR
Growth Factors
bFGF
Incubation Conditions
37°C, 5% CO2 , Subculture: enzymic
Culture Morphology
Embryonic stem cell colonial morphology
Preservation Method
Frozen / DMSO
Viability
>80%
DNA Profile
Human
Karyotype
46,XY
Characterisation Panel
Awaiting Confirmation of Characterisation Panel Tests
Sterility Test
No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen
Mycoplasma not detected by Mycoplasma PCR or Culture
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