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UK Stem Cell Bank
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KCL003-CF-1
KCL003/CF-1
Cell Line Name
KCL003/CF-1
Cell Line Status
Currently available
Cell Type
Human Embryonic
Grade
Research
Source
Human embryo / blastocyst
Source type
Cryopreserved
Consent Status
Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived
01 May 2003
Ethical Statement
In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor
Kings College London
Depositing Centre
Kings College, London
Country of Origin
United Kingdom
Depositor Contact Details
cecilia.soh@kcl.ac.uk
Journal Reference
Publications available
SCSC No.
SCSC-06-28(2)
UKSCB Accession No.
R-08-030
Distribution Bank No.
d01
Passage No.
p19
Feeder Cell Type
Mouse embryo fibroblast
Basel Medium
DMEM
Protein Source
FBS
Growth Factors
bFGF and LIF
Incubation Conditions
37°C, 5% CO2, >95% RH, Subculture: manual cutting
Culture Morphology
Embryonic stem cell colonial morphology
Preservation Method
Vitrified
Viability
>80%
DNA Profile
Human
Karyotype
46,XY CF deltaF508 mutation
Characterisation Panel
Awaiting Confirmation of Characterisation Panel Tests
Sterility Test
No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen
Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen
Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR
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