This product is a highly characterised single batch cancer genomic DNA (gDNA) International Standard covering the PIK3CA c.1633G>A (E545K) clinically-relevant variant. The standard is provided at consensus variant percentage 52.1%, and may be diluted using the WHO 1st International Standard for ATDB102 Reference Genome (18/164) or another wild-type gDNA aligned to 18/164 to generate standards at a range of variant percentages which enable the calibration of quantitative assays.
Additionally, information about the PIK3CA variant and total gene copy number per diploid human genome mass is given.
The International Standard was established by the WHO ECBS following an extensive collaborative study involving 35 laboratories across 22 countries and 5 continents and it is suitable for any NGS (Next Generation Sequencing) and dPCR (digital PCR) analysis.
NIBSC material code
Consensus variant percentage (%)
Consensus variant copy number per diploid human genome mass
Consensus total copy number per diploid human genome mass
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For more information on our International Standards for oncogene variant detection contact firstname.lastname@example.org.
Dr Ross Hawkins – Section HeadDr Ravneet BhullerMr Malcolm Hawkins Dr Leandro Lo CascioMr Noble OssaiDr Pia SanzoneMr Miltiades Stylianou
18/118: HCT 15 Cancer Genome (WHO)Other genomic reference materials
Further information on ordering genomic reference materials from NIBSC.