Equipment :
Eppendorf EDOS 5222 Liquid dipensing system
Solutions :
NHBY0/0003 vCJD Brain
NHSY0/0009 vCJD Spleen
NHBZ0/0005 Ctrl Brain
NHSZ0/00012 Ctrl Spleen
Frozen Cryopoor Human plasma (500ml bottles)
Procedure:
- Defrost plasma at 37oC.
- Prepare 1/10 dilution of Ctrl, vCJD brain in Ctrl plasma
- Prepare dilution series of each samples (Ctrl brain, vCJD
brain, Ctrl spleen, vCJD Spleen)
- Aliquot, 1ml per screw top tube. Freeze in dry ice/ ethanol 96
bath. Store at -80oC.
- Transfer onto dry ice for transport to storage facility at
NIBSC (-80 oC).
- Assemble Panel.