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UK Stem Cell Bank
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Research grade stem cell lines
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OXF2
OXF2
Cell Line Name
OXF2
Cell Line Status
Currently available
Cell Type
Human Embryonic
Grade
Research
Source
Human embryo / blastocyst
Source type
Fresh
Consent Status
Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived
02 February 2009
Ethical Statement
In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor
University of Oxford
Depositing Centre
University of Oxford
Country of Origin
United Kingdom
MUL Contact Details
Journal Reference
Publications available
SCSC No.
SCSC-10-38
UKSCB Accession No.
R-11-002
Distribution Bank No.
D01
Passage No.
p14
Feeder Cell Type
Mouse embryo fibroblast
Basel Medium
KO-DMEM
Protein Source
KO-SR
Growth Factors
bFGF
Incubation Conditions
37°C, 5% CO2, >95% RH, Subculture: Enzyme subculture
Culture Morphology
Embryonic stem cell colonial morphology
Preservation Method
freezing (10% DMSO)
Viability
>90%
DNA Profile
Human
Karyotype
46, XX
Characterisation Panel
Positive for pluripotency markers
Sterility Test
No contamination detected by EU
Pharmacopeia
Sterility Test
Mycoplasm Screen
Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen
Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR
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