Hover the "+" cursor over the dilution curve at the variant percentage required to see the dilution to be performed.
For example, to prepare a further standard of variant percentage 25% for PIK3CA c.1633G>A (E545K), material 18/118, hover the "+" cursor over 25% on the curve to see the dilution required i.e. 2.08 means that a 1 in 2.08 dilution (1 part of material 18/118 plus 1.08 parts of wild- type, material 18/164) will yield a further standard of variant percentage 25%, e.g. 2.00 μl of material 18/118 plus 2.16 μl of material 18/164.
* The variant percentage (%) is shown at 5 decimal places to ensure the accuracy of the dilution curves. Users are likely to be working with a maximum 1 or 2 decimal places so
rounding may be required.
Do not export the graphs for your calculations. Calculations are only valid when made on this webpage.