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UK Stem Cell Bank
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Research grade stem cell lines
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SHEF-2
SHEF-2
Cell Line Name
SHEF-2
Cell Line Status
Currently available
Cell Type
Human Embryonic
Grade
Research
Source
Human embryo / blastocyst
Source type
Cryopreserved
Consent Status
Donor consent was reviewed by the UK Steering Committee and was found to be consistent with the Steering Committee Code of Practice for the Use of Human Stem Cell Lines.
Date cell line derived
01 December 2003
Ethical Statement
In the opinion of the Steering Committee for the UK Stem Cell Bank and the use of Human Stem Cell Lines, the derivation of the stem cell line is consistent with UK ethical guidelines
Depositor
Pfizer
Depositing Centre
Pfizer
Country of Origin
United Kingdom
MUL Contact Details
Timothy Allsopp [
Timothy.Allsopp@pfizer.com
]
Journal Reference
Publications available
SCSC No.
SCSC-04-28-(2)
UKSCB Accession No.
R-05-028
Distribution Bank No.
D01
Passage No.
p36
Feeder Cell Type
Mouse embryo fibroblast
Basel Medium
KO-DMEM
Protein Source
KO-SR
Growth Factors
bFGF
Incubation Conditions
37°C, 5% CO2, >95% RH, Subculture: manual cutting
Culture Morphology
Embryonic stem cell colonial morphology
Preservation Method
Frozen /DMSO
Viability
50-80%
DNA Profile
Human
Karyotype
46,XX
Characterisation Panel
Confirmed SSEA-1 Low; Confirmed Positive for SSEA-3/SSEA-4, TRA-1-60, TRA-1-81, TRA-1-85, TRA-2-54, OCT3/4, SOX 2
Sterility Test
No contamination detected by EU Pharmocopeal Sterility Test
Mycoplasm Screen
Mycoplasma not detected by Mycoplasma PCR or Culture
Viral Screen
Human Virus Panel (HIV, HTLV, HBV, HCV, EBV, CMV) not detected by PCR
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